MIGEC is a software pipeline that facilitates processing and analysis of immune repertoire sequencing data generated using molecular barcoding technique. The software allows to transform raw sequencing data into human-readable clonotype table in five simple steps and is suitable for custom barcoding protocols and complex experimental layouts. Hotspot error correction algorithm implemented in MIGEС yields robust and nearly error-free results compared to a straightforward barcode-based data analysis.
Combination of MIGEC and MiXCR software allows for a high quality full-length profiling of IG repertoires.
Please see documentation for details.
Cite the tool as:
Shugay M et al. Towards error-free profiling of immune repertoires. Nature Methods 11, 653–655 (2014) doi: 10.1038/nmeth.2960.
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