The Human TCR Kit is designed for unbiased amplification of TCR alpha and beta cDNA libraries using 5’RACE with unique molecular identifiers (UMI) incorporated within template switch oligo. 5’RACE UMI technique allows for exact quantification of template cDNA molecules, control for input bottlenecks, and accurate normalization of samples for comparisons of repertoire diversity metrics.
The kit includes a set of oligonucleotides sufficient to prepare 192 TCR alpha and 192 TCR beta cDNA libraries starting from 192 RNA samples.
The kit allows to start with RNA derived from 100 to 1 million sorted/purified T cells, from peripheral blood leukocytes, or from T cell-containing tissues, and produces indexed ready-to-sequence-on-Illumina® libraries. Up to 96 samples can be processed in parallel.
Currently we recommend that you use our MIGEC™, MiXCR™ and VDJtools™ software for analysis of the data obtained with the kit. Note that commercial use of MIGEC™, MiXCR™ and VDJtools™ software requires licensing from MiLaboratory LLC: please email us at firstname.lastname@example.org.
Please contact us with any questions related to the kit and data analysis: email@example.com.